The New Way to Optimize Hybridoma Cell Culture
Problems culturing hybridoma cells are common.
These problems slow down product development and biomanufacturing.
But, there are solutions!
Hybridoma cells are engineered hybrid cells which are extensively used to produce large amounts of monoclonal antibodies. The antibodies produced in hybridoma cell lines are used in a wide range of applications in the biomedical and research industries, including in R&D and diagnostics.
Problems Faced When Culturing Hybridoma Cell Lines
Traditionally, hybridoma cell culture media contained high levels of animal-derived serums or animal protein components. However, due to IgG contamination and inconsistent performance from animal-origin components, most researchers and biomanufacturing applications switched to serum-free, animal-free or chemically-defined media formulations. This resulted in slower cell growth and long adaptation timelines.
The Solution: Media Supplements that Boost Performance
InVitria has focused its product development on maximizing hybridoma performance by developing defined, IgG-free media supplements. These products help you overcome the challenges when working in chemically-defined, animal-free, serum-free and classical media so that you can get the most out of your hybridoma cell lines. These supplements are cost effective compared to supplementation with serum or expensive growth cocktails.
Cellastim (recombinant albumin)
Optiferrin (recombinant transferrin)
With these supplements, you can solve the most challenging aspects of in vitro culturing of hybridoma cells.
- Improve consistency with defined supplements and reduce or eliminate FBS use.
- Simplify purification with IgG-free supplements.
- Improve hybridoma growth, leading to shorter doubling times.
- Increase antibody yield
- Increase viability
Cells grew to higher density in commercial media when supplemented with Zap-Hybridoma. Similar growth kinetics were observed with other leading commercial media when they were supplemented with Zap-Hybridoma.
Hybridoma cells were grown in two media either without supplementation (yellow), or supplemented with plasma-derived human serum albumin (pHSA)(red), Cellastim (blue) or 10% FBS (black). Increased antibody productivity was observed in the media supplemented with Cellastim at 1 g/L.
Shown is the viable cell density of Sp2/0 Hybridoma culture on day 4 following withdrawal from media supplemented with 10% FBS. After adaptation to media supplemented with 10% FBS, cells were washed and transferred to leading CD media at 0.045 x106 cells/mL with Zap-Hybridoma, 10% FBS, or no supplement.
In large scale production of purified antibodies, a clearly defined and consistent production process is the key.
If you do not have the time to optimize your hybridoma cell culture media, let InVitria help! We can tailor any media to optimally meet your needs.
Click here for our media optimization services.
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