ITSE Animal-Free is a cell culture media supplement containing recombinant insulin and transferrin formulated with selenium and ethanolamine for improved serum reduced or serum free cell growth.

ITSE Animal-free replaces insulin and transferrin which contain animal derived ingredients.

ITSE Animal-Free is a recombinant insulin and transferrin cell culture media supplement that is made in the USA and is animal component free. It is commonly used to reduce or remove fetal bovine serum. ITSE Animal-Free is unique in that it uses recombinant technology, which allows for consistent results. ITSE Animal-Free provides all these benefits and is more cost-effective than traditional insulin and transferrin supplements.

Zap-Hybridoma is an animal-free, recombinant cell culture media supplement that is defined and consistent from lot-to-lot. Zap-Hybridoma is also bovine IgG-free. This eliminates IgG contamination and leads to improved quality of the final antibody because there is no contamination with bovine IgG resulting from the use of serum.. In some cases low IgG serum is used to address this issue, but low IgG serum adds significant expense and is not completely IgG free. Use of low IgG serum can double the cost of cell culture media.

What customers are saying about ITSE Animal-Free:

"InVitria’s ITSE Animal-Free works well, and better than our alternative product from another supplier."

Senior Cell Culture Scientist, Ludwig Institute for Cancer Research

Insulin, transferrin, selenium and ethanolamine are typically required for optimal cell growth in serum-free media (1). Ethanolamine is a phospholipid precursor that improves the performance of cells in serum-free media (2) and is required for the growth of some cell types (3). Insulin has cell signaling functions and promotes the uptake of glucose and amino acids (4). Transferrin is a universal source of iron for cells that is non-toxic carrier of iron and reduces the generation of toxic free-radicals and peroxide (5). Selenium is required for the activity of glutathione peroxidase, thioredoxin reductase, and other antioxidant enzymes (6).

Advantages:

• Cost-effective
• Recombinant proteins improve consistency
• Eliminate animal components in serum free cell culture
• Ready-to-use 100x liquid formulation that is filled to maintain sterility

Cell Culture Media Comparisons

All the performance,
none of the animal-derived components

Vero cells were grown over four days in DMEM/F12 media supplemented with InVitria’s ITSE Animal-Free and another commercial ITSE. There is no significant difference between the performance cells grown in media supplemented with ITSE Animal-Free and cells grown in another commercial ITSE. Cells were grown in triplicate 1 mL static cultures, and were seeded at 1.2x104 cells/well.

For more information, please call 1-800-916-8311 or email us