Applications in Microbial Fermentation

Lysis doesn’t always mean lower protein recovery. Recombinant protein recovery from microbial fermentation requires that your cell lysis methods optimize both the quality and quantity of the proteins you are aiming to recover.Other microbial cell lysis methods such as chicken lysozyme or mechanical cell lysis pose several issues:

Chicken Lysozyme

  • Varying animal sources leads to lot-to-lot inconsistency
  • Contamination of recovered proteins
  • Problematic duplication of results

Mechanical Cell Lysis

  • Leads to denaturing due to physical shearing
  • Time consuming
  • Requires adding an expensive step to your recovery process

InVitria has designed a new E. coli cell lysis reagent, Lysobac (recombinant human lysozyme) that specifically addresses these common problems. Unlike other cell lysis reagents used in E. coli lysis after microbial fermentation, this new reagent is not made from animal products.

Typical Problems

InVitria Cell Lysis Reagents

Animal-origin Contamination of Recombinant Protein

Animal-free

Protein denaturation due to shearing

Gentle Lysis with no denaturing of recombinant protein

Poor Recovery

Higher Protein Recovery

Poor reagent consistency

Lot-to-lot consistency

Multi-step process

Microbial cell lysis in a single step

Related Products

Lysobac

Recombinant human lysozyme – 4 times more active than commonly used egg white-derived products in a one-step process.

Learn about Lysobac