Insulin supports cell proliferation and viability
Insulin in Cell Culture
Insulin is a critical component in most serum-free media formulations. Insulin supports cell growth and regulates the cellular uptake and utilization of glucose, amino acids, and lipids. Insulin also has anti-apoptotic properties. Insulin is a 2-chain, ~5.8 KDa polypeptide hormone that is produced by the β-cells of pancreatic islets. The combination of insulin and transferrin allows the concentration of serum to be reduced in many cell culture applications.
Other Insulin Information
High quality animal-free human insulin has mostly replaced blood-derived bovine insulin in cell culture and bioprocessing applications. Insulin is typically added to cell culture media at concentrations ranging from 1 to 10 milligrams per liter of medium, which is roughly 100-fold the physiological concentration. At these concentrations, Insulin can mediate a broader range of beneficial effects by interacting with both the insulin and IGF-1 (Insulin-like Growth Factor) cellular receptors. These effects include the inhibition of cell apoptosis (death) that is caused by the withdrawal of serum.
Recombinant insulin and transferrin cell culture media supplement formulated with selenium and ethanolamine for improved cell growth and productivity. Animal component free.
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