Lactoferrin promotes cell growth and protects cells from apoptosis in the body and as a supplement in cell culture media.
Lactoferrin in Cell Culture
In cell culture, lactoferrin is added to cell culture media to increase cell growth and productivity. Lactoferrin also protects cells from apoptosis (programmed cell death) and promotes cell viability. It can bind iron and has been used in some cell culture applications to deliver iron to cells without causing free radical molecules that are typically formed when iron chelators or other sources of iron are delivered to cell culture. Oxidative reactions from excess iron in cell culture media leads to free radical molecules that can cause damage to cells. Lactoferrin often outperforms and is used as a replacement for more expensive Insulin Like Growth Factor (IGF). Lactoferrin has been shown to increase cell growth and productivity in several cell types including stem cells, primary cells and CHO cells. .
Lactoferrin is available as an animal-free, recombinant cell culture media supplement, which eliminates safety concerns associated with supplements that are animal or human sourced. Recombinant lactoferrin also provides the advantage of being consistent across product lots unlike animal sourced or undefined supplements. Lacromin, produced in an animal-free, recombinant system (See ExpressTec) provides all the benefits of lactoferrin, without the inconsistency or safety concerns
Other Lactoferrin Information
Lactoferrin is a multi-functional protein and has many jobs in the body. In addition to the benefits to cell growth and viability mentioned above, lactoferrin also act as an antimicrobial in the body. Lactoferrin protects the body against bacteria, viruses, fungi and other infectious microorganisms. It can also inhibit the binding of bacteria or viruses to host cells, thus preventing infections. Lactoferrin’s ability to bind and carry iron without generating free radicals is one way that lactoferrin functions as an antioxidant to protect the body and also deliver iron to cells, this function is one of lactoferrin’s primary roles in breast milk. Lactoferrin is also sometimes used as an adjuvant for certain vaccines.
Lactoferrin also has many direct human health applications including incorporation in clinical nutrition products, cosmetics, wound care formulations, infant formula and oral hygiene products.
Recombinant human lactoferrin – CHO growth factor that outperforms insulin and IGF-1 increasing antibody production.
- Baker EN, Baker HM. (2009). A structural framework for understanding the multifunctional character of lactoferrin. Biochimie, 91(1):3-10.
- Conneely OM. (2001). Antiinflammatory activities of lactoferrin. Journal of the American College of Nutrition, 20(90005):389S-395S.
- Flanagan JL, Willcox MD. (2009). Role of lactoferrin in the tear film. Biochimie, 91(1):35-43.
- Gonzalez-Chavez SA, Arevalo-Gallegos S, et al. (2009). Lactoferrin: structure, function and applications. Int J Antimicrob Agents, 33(4):301e1-8.
- Jenssen H, Hancock RE. (2009). Antimicrobial properties of lactoferrin. Biochimie, 91(1):19-29.
- Lonnerdal B. (2009). Nutritional roles of lactoferrin. Curr Opin Clin Nutr Metab Care, 12(3):293-7.
- Mohan P, Abrams SA. (2009). Oral lactoferrin for the treatment of sepsis and necrotizing enterocolitis in neonates. Cochrane Database Syst Rev, (1):CD007138.
- Ochoa TJ, Cleary TG. (2009). Effect of lactoferrin on enteric pathogens. Biochimie, 91(1):30-4.
- Paesano R, Pietropaoli M, et al. (2009). The influence of lactoferrin, orally administered, on systemic iron homeostasis in pregnant women suffering of iron deficiency and iron deficiency anaemia. Biochimie, 91(1):44-51.
- Pierce A, Legrand D, Mazurier J. (2009). Lactoferri: A multifunctional protein. Médecine Sciences, 25(4):361-9.
- Puddu P, Valenti P, et al. (2009). Immunomodulatory effects of lactoferrin on antigen presenting cells. Biochimie, 91(1):11-8.
- Rodrigues L, Teixeira J, Schmitt F, Paulsson M, Helena LM. (2009). Lactoferrin and cancer disease prevention. Critical Reviews in Food Science and Nutrition, 49(3):203-17.
- Tomita M, Wakabayashi H, et al. (2009). Twenty-five years of research on bovine lactoferrin applications. Biochimie, 91(1):52-7.