Cellastim Guidelines for Use

Recombinant Human Serum Albumin
Cell culture grade
Animal-free
Immunoglobulin free

Introduction

Cellastim is a recombinant human serum albumin (HSA) that has been produced from an animal-free expression system. Cellastim has the identical amino acid sequence as the major HSA isoform found in human serum. Albumin is a cell culture supplement that functions as a carrier protein for fatty acids, growth factors, and trace minerals. Albumin also protects cells from toxins and physical shear. Albumin is used to reduce or eliminate the need for serum and to maximize cell growth, viability, and productivity in classical, commercial, and custom media.

The optimum concentration of albumin for cell culture applications varies with the type of cell and the composition of the medium. Albumin is typically used at concentrations of 0.5 to 2 g/L in media formulations.

Storage

Recommended storage at -20°C, tightly sealed, and protected from light.

Instructions for use

Prepare concentrated liquid stocks of Cellastim by dissolving in cell culture grade PBS or water. Use gentle mixing or inversion to minimize the formation of bubbles. Allow Cellastim to dissolve for at least 1 hr. Liquid stocks should be sterile filtered prior to use. InVitria recommends a pre-filtration step with a 0.8 µm filter prior to a final sterile filtration step with a 0.2 µm filter.

InVitria recommends the adaptation of cells to Cellastim-supplemented medium for a couple of passages to insure optimum performance. Following the adaptation, the end user can determine the optimum concentration of Cellastim for their particular purpose. Cells previously adapted to a medium containing albumin typically require little adaptation to Cellastim.

For further information on Cellastim contact InVitria technical support at 1-800-916-8311.

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