Leukemia Inhibitory Factor (rhLIF) Guidelines for Use

rhLIF (Recombinant Human Leukemia Inhibitory Factor)
Animal-Free, Chemically Defined
Cat. Number: 777LIF048

Introduction

Leukemia Inhibitory Factor is a glycosylated multifunctional 20 kDa member of the interleukin-6 type cytokines (1). LIF-mediated binding of the LIF receptor (LIFR) and co-receptor gp130 induces the JAK/STAT3, PI3K/AKT and MAPK signaling cascades (2,3). LIF signaling has been implicated in mammalian embryo implantation (3), regulation of early hematopoietic stem cells (4), regulation of adult human T cell fates (5), and maintenance of embryonic stem cell pluripotency (6). Human LIF demonstrates a 78% homology with mouse LIF and has been shown to compete in vitro with murine LIF binding on M1 leukemia cells (7).

Reagent Supplied and Storage

Recombinant human LIF (rhLIF) is produced using the plant-based ExpressTec expression system which utilizes no animal components in manufacturing. rhLIF is supplied as a 0.2µm filtered, frozen, concentrated stock solution at 500 μg/mL in buffered saline with 0.02% TWEEN©20. Concentrated stock should be stored in -80º C. The product can be diluted to 10 μg/mL, a concentration typically utilized for sale and distribution. The solution 10ug/mL can be stored at 2-8º C for 24 months.

Instructions for use

Concentrated stock rhLIF should be thawed as quickly as possible by partial immersion in a water bath at room temperature (~22º C) with periodic gentle swirling. Monitor the thawing process visually and place vial on ice or at 2-8º C when fully thawed. rhLIF is suitable for cell culture applications. rhLIF should be diluted in tissue culture medium to the desired concentration. We recommend the use of rhLIF at 10-20ng/mL; however, the optimal concentration is dependent upon cell type and should be determined experimentally.

References

  1. Mathieu, M., et al. Stem Cell Rev., 8, 1 (2012).
  2. Hirai, H., et al. Biochem J., 438, 11 (2011).
  3. Dimitriadis, E., et al., Placenta 31., 99 (2010).
  4. Leary, AG., et al., Blood., 75, 1960 (1990).
  5. Metcalfe, S., Genes Immun., 12, 157 (2011).
  6. Smith, A., et al., Dev Biol., 151, 339 (1992).
  7.  Gough, N., et al., Proc. Nathl. Acad. Sci. USA., 85, 2623 (1988).

For further information on rhLIF contact InVitria technical support at 1-800-916-8311.

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