Zap-SR Serum Reducer
Animal-Free, Chemically Defined, Sterile-filtered
Catalog Number 777ZAP053
Storage Conditions: 2-8ᵒC tightly sealed, avoid unnecessary air and light exposure
InVitria’s Zap-SR supplement is designed to reduce serum in cell culture medium while maintaining cell proliferation and high cell viability. Zap-SR is compatible with F12, DMEM/F12, DMEM, IMDM, and other basal medium formulations. The ingredients of Zap-SR do not contain any animal-derived components and is prepared as a sterile-filtered 100x concentrate.
Instructions for Use
Requirements for extracellular matrix, growth factors, and other serum-derived components are highly specific for individual cell lines. Therefore, we recommend an initial serum reduction study per desired Zap-SR application as specified below in Table 1. Cells should be passaged directly from the 10% serum-containing medium into the reduced serum medium with and without Zap-SR and standard subculture times and protocols should be followed. The highest concentration of serum that fails to support adequate cell growth without Zap-SR but is rescued by the addition of Zap-SR is the optimum FBS/Zap-SR mixture. Further reductions in serum can be performed as desired.
|Media Formulation||mL Reagent/Liter Complete Medium|
|FBS (mL)||Zap-SR (mL)|