Cell Line: VERO

VERO Expansion in the iCellis® Bioreactor with Chemically Defined and Blood Free Virus Production Media

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Introduction Manufacturing vaccines for both preventative and therapeutic applications requires the production of  large amounts of virus from host cell substrates. Increased demand for these viral products has induced a major shift in the model of viral vaccine manufacturing from more primitive platforms, such as embryonated chicken eggs (ECE), to more robust large-scale methods that …

Emergence of Blood-Free Recombinant Human Albumin

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Emergence of Blood-Free rHSA Albumin is a naturally occurring protein in animals and humans responsible for numerous cellular functions and is integral to the manufacturing of biopharmaceuticals. Various benefits of albumin inclusion in upstream manufacturing, downstream processing, and final formulation applications will be discussed in detail but can be briefly summarized as: Serves as an …

OptiVERO – Virus Production Using VERO Cells in Flasks without Serum in Chemically Defined Cell Culture Media Application Note

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Workflow Summary Combine kit supplements of OptiVERO. Prepare complementary reagents. Wash cells to remove traces of FBS and harvest from growth surface. Seed in OptiVERO and grow to confluence. Add desired virus to be expanded in minimal basal EMEM. Incubate for 2 hours at 37°C. Add fresh OptiVERO. Allow virus expansion for 3-13 days. Quantify …